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Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
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Immunology and Infection
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JoVE Journal Immunology and Infection
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

DOI:
10.3791/54599-v

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07:14 min

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October 18, 2016

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, , , , , , , , , ,
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz,Autonoma University, 2Department of Physiology, Faculty of Medicine,Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa,Instituto de Salud Carlos III (ISCIII)

Chapters

  • 00:05Title
  • 00:51Kidney Perfusion and Extraction
  • 02:07Kidney Digestion and Single Cell Suspension
  • 03:13Cell Staining and Flow Cytometry
  • 04:29Results: Representative Rat Peritoneal Macrophage Characterization
  • 05:53Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes a detailed protocol for phenotypic and quantitative analysis of resident macrophages from rat kidneys by flow cytometry. The resulting stained cells can be also used for other applications, including cell sorting, gene expression analysis or functional studies, thus increasing the information obtained in the experimental model.

Tags

MacrophagesKidneyFlow CytometryCharacterizationPhenotypeM1M2InflammationRenal Disease

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