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Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
DOI:

11:36 min

February 10, 2017

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Chapters

  • 00:05Title
  • 00:47Treatment of Flies and Imaging Brains
  • 02:54Image Analysis: Generating the Spots, Surface Objects, Start-points, and End-points
  • 08:04Image Analysis: Calculating the Number of Spots, Distribution of Spot Frequency and Enrichment Level of Brp-GFP
  • 09:48Results: Brp-GFP Expression in R8 Synapses of the Medulla
  • 11:02Conclusion

Summary

Automatic Translation

Here we show how to quantify the number and spatial distribution of synaptic active zones in Drosophila melanogaster photoreceptors, highlighted with a genetically encoded molecular marker, and their modulation after prolonged exposure to light.

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