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A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

DOI:
10.3791/55191-v

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09:49 min

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January 14, 2017

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, ,
1Wuhan Institute of Virology,Chinese Academy of Sciences, 2Genetic Resources Center, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences

Chapters

  • 00:05Title
  • 01:36Preparation of Epstein Barr Virus
  • 03:32Treatment of Whole Blood with Red Blood Cell Lysis Buffer and Epstein Barr Virus Infection
  • 05:29Expansion and Cryopreservation of B-LCLs
  • 07:27Results: B Lymphoblastoid Cell Viability Following Red Blood Cell Lysis
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

A simple red blood cell lysis method for establishing B-LCLs was developed with high immortalization efficiency, requiring a small amount of blood and saving time from initiation to cryopreservation.

Tags

B Lymphoblastoid Cell LinesB-LCLsImmortalization EfficiencyRed Blood Cell LysisEpstein-Barr VirusPBMCDensity Gradient CentrifugationEBV TransformationCell CultureCryopreservationVirus Particle ReleaseFiltrationWhole Blood

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