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Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation
JoVE Journal
Medicine
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JoVE Journal Medicine
Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation
DOI:

08:05 min

July 03, 2017

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Chapters

  • 00:05Title
  • 00:54Isolation and Labeling of Erythrocytes (ICG) and Leukocytes (Sodium Fluorescein)
  • 03:25Live-cell Imaging of Labeled Erythrocytes
  • 05:26Image Analysis Using MtrackJ Software
  • 06:56Results: Visualizaing the Labeled Erythrocytes and Leukocytes
  • 07:38Conclusion

Summary

Automatic Translation

Live-cell imaging of the labeled blood cells in ocular circulation can provide information about inflammation and ischemia in diabetic retinopathy and age-related macular degeneration. A protocol to label blood cells and image the labeled cells in the retinal circulation is described.

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