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Studying Diabetes Through the Eyes of a Fish
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Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

Article DOI: 10.3791/56674-v • 10:07 min • December 26th, 2017
December 26th, 2017 •
1, 2, 2, 2, 1
1Department of Vascular Biology and Tumorangiogenesis, Center for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim, Heidelberg University, 2V. Medical Clinic, Medical Faculty Mannheim, Heidelberg University

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Here, we discuss a method protocol which will allow an easy analysis of the adult tg(fli:EGFP) zebrafish retinal vasculature as a fast read-out in settings of long-term vascular pathologies linked to neoangiogenesis and structural changes.

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Diabetes Fish Microdissection Visualization Analysis Adult Zebrafish Retinal Vasculature Vascular Pathologies Neoangiogenesis Structural Changes Fluorescence Microscopy Laser Scanning Confocal Microscopy Diabetic Retinopathy Retinovasculature Intervascular Fluorescence PFA PBS Solution Euthanizing Adult Zebrafish Scalpel Fixative Agarose Petri Dish
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