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Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature
JoVE Journal
Medicine
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JoVE Journal Medicine
Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

DOI:
10.3791/56674-v

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10:07 min

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December 26, 2017

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, , , ,
1Department of Vascular Biology and Tumorangiogenesis, Center for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim,Heidelberg University, 2V. Medical Clinic, Medical Faculty Mannheim,Heidelberg University

Chapters

  • 00:05Title
  • 00:54Fixation of Zebrafish Tissue
  • 01:41Preparation of the Zebrafish Retinal Vasculature
  • 06:39Mounting and Visualization of the Retinal Vasculature
  • 07:51Results: Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we discuss a method protocol which will allow an easy analysis of the adult tg(fli:EGFP) zebrafish retinal vasculature as a fast read-out in settings of long-term vascular pathologies linked to neoangiogenesis and structural changes.

Tags

Keywords: Tg(fli:EGFP) ZebrafishRetinal VasculatureMicrodissectionVisualizationAnalysisDiabetic RetinopathyMicrovascular ComplicationsFluorescence MicroscopyConfocal MicroscopyPFA FixationAgarose EmbeddingEye DissectionCorneal PunctureLens Extraction

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