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An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
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Immunology and Infection
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JoVE Journal Immunology and Infection
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

DOI:
10.3791/55559-v

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12:17 min

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May 08, 2017

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, , , , ,
1Public Health Research Institute Centre, New Jersey Medical School,Rutgers, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID,NIH

Chapters

  • 00:05Title
  • 01:02In Vitro Generation of Caseum Surrogate from THP-1 Cells
  • 05:23Rapid Equilibration Dialysis (RED) Assay
  • 08:02LC-MS Quantification and Data Analysis
  • 09:36Representative RED Assay Results
  • 11:27Conclusion

Summary

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Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

Tags

In Vitro Caseum Binding AssayDrug PenetrationTuberculosis LesionsAntimicrobial PenetrationTHP-1 MonocytesIliac AcidPMACell CultureRPMI Medium

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