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An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
DOI:

12:17 min

May 08, 2017

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Chapters

  • 00:05Title
  • 01:02In Vitro Generation of Caseum Surrogate from THP-1 Cells
  • 05:23Rapid Equilibration Dialysis (RED) Assay
  • 08:02LC-MS Quantification and Data Analysis
  • 09:36Representative RED Assay Results
  • 11:27Conclusion

Summary

Automatic Translation

Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

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