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Journal / Developmental Biology / Förberedelse av medelstora
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Developmental Biology

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JoVE Journal Developmental Biology
Förberedelse av medelstora
Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
Journal (Developmental Biology)
Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
Journal (Developmental Biology)
Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
 
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Förberedelse av medelstora

Article DOI: 10.3791/55804-v • 09:16 min • May 30th, 2017
May 30th, 2017 •
1, 1, 1, 2, 1
1Biology Department, University of Massachusetts Boston, 2Department of Bioengineering, University of California, San Diego

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Målet med detta protokoll är att tillhandahålla ett enkelt och billigt tillvägagångssätt för att samla Drosophila embryon i medium skala (0,5-1 g) och förbereda proteinextrakt som kan användas i nedströms proteomiska applikationer, såsom affinitetsrenings-masspektrometri (AP-MS ).

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Utvecklingsbiologi utgåva 123 extrakt lysat protein, Fruktfluga embryo proteomics medium skala rening bur
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