Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Modellering neuronala dödsfall och Degeneration i …
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Modellering neuronala dödsfall och Degeneration i mus primära cerebellär Granule nervceller
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
Journal (Neuroscience)
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal
Journal (Neuroscience)
Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal
 
Click here for the English version

Modellering neuronala dödsfall och Degeneration i mus primära cerebellär Granule nervceller

Article DOI: 10.3791/55871-v • 10:36 min • November 6th, 2017
November 6th, 2017 •
1,2, 1, 3, 1,2,4
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Integrated Program in Neuroscience, McGill University, 3Department of Cellular and Molecular Medicine, University of Ottawa, 4Department of Oncology, Faculty of Medicine, McGill University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Det här protokollet beskriver en enkel metod för att isolera och odla primära mus cerebral granule nervceller (CGNs) från 6-7 dag gammal PUP, effektiv cellsignalering av CGNs för förlust och vinst av funktion studier, och modellering NMDA-inducerad neuronala excitotoxicitet, låg-kalium-inducerad celldöd, DNA-skador och oxidativ stress med hjälp av samma kultur-modellen.

Tags

Neurovetenskap frågan 129 lillhjärnan cerebellär Granule nervceller (CGN) Adenovirus Lentiviral transduktion excitotoxicitet N-metyl-D-aspartat (NMDA) apoptos kaliumklorid (KCl) Camptothecin väteperoxid (H2O2)
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter