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Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
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1Departments of Pathology and Infectious Diseases and Microbiology,University of Pittsburgh, 2University of Pittsburgh Cancer Institute, 3Department of Environmental and Occupational Health,University of Pittsburgh, 4Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences,University of Pittsburgh
Chapters
- 00:05Title
- 00:46Genomic DNA Digestion
- 01:39Genomic DNA Gel Electrophoresis
- 02:56Gel Fluorescent Imaging and Hybridization
- 05:17Hybridization with Telomere Probe
- 07:36Gel Washing, Phospher Screen Exposure, and Radio Imaging
- 09:08Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis
- 10:28Conclusion
In this article, a detailed protocol for quantifying telomere length using a modified terminal restriction fragment analysis is discussed that provides fast and efficient direct measurement of telomere length. This technique can be applied to a variety of cell sources of DNA for quantifying telomere length.
