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Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
JoVE Journal
Medicine
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JoVE Journal Medicine
Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
DOI:

08:36 min

August 31, 2017

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Chapters

  • 00:05Title
  • 00:41Day 1: Primary Immunostaining of Mouse Muscle Sections
  • 01:57Day 2: Secondary Immunostaining of Mouse Muscle Sections
  • 03:24Collecting Muscle Images and their Analysis with the Fiber Quantification Macro
  • 06:23Results: Representative Immunostaining and Morphometric Analysis
  • 07:50Conclusion

Summary

Automatic Translation

Immunohistochemical staining of myosin heavy chain isoforms has emerged as the state-of-the-art discriminator of skeletal muscle fiber-type (i.e., type I, type IIA, type IIX, type IIB). Here, we present a staining protocol along with a novel semi-automated algorithm that facilitates rapid assessment of fiber-type and fiber morphology.

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