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Using the Dot Assay to Analyze Migration of Cell Sheets
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Cancer Research
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JoVE Journal Cancer Research
Using the Dot Assay to Analyze Migration of Cell Sheets

Using the Dot Assay to Analyze Migration of Cell Sheets

DOI:
10.3791/56451-v

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09:42 min

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December 05, 2017

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1Laboratory of Cellular and Molecular Biology, National Cancer Institute,National Institutes of Health

Chapters

  • 00:05Title
  • 00:47Coating Dishes (Day 1)
  • 01:55Preparing Cell Suspension for Dot Assay (Day 2)
  • 03:11Plating Cells
  • 04:22Stimulating Cells (Day 3)
  • 05:19Hematoxylin-Eosin Staining to Measure Colony Growth (Days 4-7)
  • 06:06Time Lapse Microscopy
  • 07:12Results: Analysis of Migration of Cells Sheets Using the Dot Assay
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

Cell migration is essential for development, tissue maintenance and repair, and tumorigenesis, and is regulated by growth factors, chemokines, and cytokines. This protocol describes the dot assay, a two-dimensional, unconstrained migration assay to assess the migratory phenotype of attached, cohesive cell sheets in response to microenvironmental cues.

Tags

Dot AssayCell MigrationCell MotilityCell SheetsMicroenvironmental CuesCancerCytokinesGrowth FactorsCell StainingCell ScatteringImmunostainingProtein AnalysisDNA AnalysisRNA AnalysisAnimal InjectionMouse Collagen IVCell SuspensionTrypsin EDTACell CountingCell Plating

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