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An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration
JoVE Journal
Behavior
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JoVE Journal Behavior
An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration
DOI:

06:52 min

September 12, 2017

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Chapters

  • 00:05Title
  • 01:14Fly Collection
  • 02:13Automated RING Assay
  • 03:56RING Assay Data Analysis
  • 05:19Results: Adult Climbing Behavior in Drosophila melanogaster
  • 06:03Conclusion

Summary

Automatic Translation

This step-by-step protocol analyzes Drosophila negative geotaxis behavior using an automated multi-cylinder system that hosts hundreds of flies and synchronizes their action by an electric motor. Upon synchronization, fly negative geotaxis behavior is assayed, digitally recorded, and analyzed using the self-designed RflyDetection software.

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