Isolation and Cultivation of Adult Rat Cardiomyocytes

Published 10/19/2017
0 Comments
  CITE THIS  SHARE 
Medicine

Your institution must subscribe to JoVE's Medicine section to access this content.

Fill out the form below to receive a free trial or learn more about access:

Welcome!

Enter your email below to get your free 10 minute trial to JoVE!





By clicking "Submit", you agree to our policies.

 

Summary

Here, we present a protocol for the isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). Isolated ARVC can be used for short and long-term cultivation. The isolation and cultivation of ARVC can play a key role in developing new treatment regimens for cardiac diseases.

Cite this Article

Copy Citation

Nippert, F., Schreckenberg, R., Schlüter, K. D. Isolation and Cultivation of Adult Rat Cardiomyocytes. J. Vis. Exp. (128), e56634, doi:10.3791/56634 (2017).

Abstract

In an intact heart, adjacent cells influence adult cardiomyocytes. With the method of isolation and cultivation of adult cardiomyocytes, a precise investigation of the behavior of these cells under specific treatments and environments is possible. This manuscript presents a protocol for successful isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC).

The rat is sacrificed by cervical dislocation under deep anesthesia. Then, the heart is extracted and the aorta is uncovered. Subsequently, perfusion on the Langendorff perfusion system with calcium depletion and collagenase treatment is performed. Afterwards, ventricular tissue gets minced, re-circulated, and filtered, followed by three centrifugation steps with gradual addition of CaCl2 until physiological calcium concentration is reached. ARVC are plated on cell culture dishes. After refreshing the cell culture medium, ARVC can be cultivated for up to six days without changing the serum-containing culture medium. Isolation of ARVC is a calcium sensitive process. Small changes in the intracellular calcium concentration cause a decrease in the quality and viability of the isolated cells.

Freshly isolated ARVC are rod shaped. Within the first days of cultivation they lose the rod-shaped morphology and form pseudopodia-like structures (spreading). During this morphological formation ARVC initially degrade their contractile elements followed by a reformation through actin stress fibers and de novo sarcomerogenesis. After one week of cultivation, most ARVC show a widespread appearance with a clearly detectable cross striation. This process is sensitive to intracellular calcium concentration, as treatment with ionomycin attenuates spreading. Key markers in this process of de- and re-differentiation are β-myosin heavy chain (β-MHC), oncostatin M (OSM), and swiprosin-1 (EFHD2). Recent studies have suggested that cardiac re- and de-differentiation occurring under culture conditions mimics features seen in vivo during cardiac remodeling. Therefore, isolation and cultivation of ARVC play a key role in understanding the biology of cardiomyocytes.

Introduction

Adult cardiomyocytes in vivo work as an electrical syncytium based on cell-cell contacts between myocytes. In addition, they are influenced by adjacent cells like cardiac fibroblasts, endothelial cells, neurons, and inflammatory cells1. In order to study the ability of cardiomyocytes to adapt their intracellular organization to altered load conditions, as seen during cardiac hypertrophy, which is an initial step leading to heart failure, the isolation and cultivation of adult ventricular rat cardiomyocytes (ARVC) is necessary2,3,4. Historically, cardiomyocytes were first isolated from embryonic chick hearts5,6. A few years later, the first isolation of terminally differentiated cardiomyocytes was described by using calcium depletion7. However, these adult cardiomyocytes were not calcium tolerant and could therefore not be used for functional assays. Finally, in 1976 a new protocol enabled Powell and Twist to investigate adult ventricular cardiomyocytes under physiological conditions8. As a first step, they isolated adult cardiomyocytes under low calcium concentrations and thereafter increased calcium to physiological concentrations in a stepwise procedure. Today, most protocols for the isolation and cultivation of adult cardiomyocytes work with this calcium protocol and use collagenase for the enzymatic digestion of the dense cell-cell contacts1.

For a successful cultivation, fetal calf serum (FCS) or oncostatin M (OSM) is required. ARVC perform a de- and re-differentiation with extensive structural changes including sarcomere disassembly and reformation9,10,11,12. This process is accompanied by a re-expression of fetal-type genes, like β-myosin heavy chain (β-MHC), as known from hypertrophy, and a formation of pseudopodia-like structures, also called spreading4,11,13. Furthermore, swiprosin-1 (EFHD2), a newly identified protein, plays a major role in the process of re-differentiation of cultivated ARVC11. As a result, ARVC in culture transform into widespread, polymorphic cells, which spontaneously show contractions after two to three weeks in culture2,4,14.

Recent discoveries have revealed that cardiac re- and de-differentiation as it occurs under culture conditions mimics features seen in vivo during cardiac remodeling10,15. Cardiac remodeling is a key process during cardiac diseases16. As cardiac diseases are still the main cause of death in industrialized societies, a better understanding of the biology of adult cardiomyocytes is important (WHO; 2015). Isolation and cultivation of ARVC can help to develop new strategies and medicines for the treatment of cardiac diseases. With this manuscript, a protocol for the isolation and cultivation of ARVC is provided. Furthermore, some critical parts of this method are highlighted in the discussion section.

Subscription Required. Please recommend JoVE to your librarian.

Protocol

The investigation is conducted according to the Guide for the Care and Use of Laboratory Animals published by the US National Institute of Health (NIH Publication No. 85-23, revised 1996). In general, male wistar rats aged 3 to 4 months and with an average weight of 250 - 350 g are used for this protocol. One rat heart is sufficient for 20 culture dishes (1 mL per dish; inner diameter: 35 mm) with an approximate cell density of 1.5 x 104 cells/1000 mm2.

1. Preparation of Media and Reagents

  1. Creatine-carnitine-taurine medium (CCT medium)
    NOTE: CCT medium is a complex medium based on medium 199 with the addition of creatine, carnitine, and taurine.
    1. Prepare 1 L of medium 199: add 3.6 g Hepes and mix for 1 h. Then add 655.5 mg creatine (5 mM), 395.4 mg carnitine (2 mM), and 625.5 mg taurine (5 mM). Carnitine and taurine change pH to <7. In order to inhibit the growth of any contaminating cells, e.g. endothelial cells or fibroblasts, add 10 µM cytosine β-D-arabinofuranoside to the medium. Adjust the pH with NaOH (2 mM) to 7.4 and sterile filter the medium. Store the CCT medium at 4 °C.
  2. Powell medium
    1. For 1 L Powell medium, dissolve 6.43 g NaCl (110 mM) with 0.19 g KCl (2.5 mM), 0.16 g KH2PO4 (1.2 mM), 0.3 g MgSO4 7H2O (1.2 mM), 5.96 g Hepes (25 mM), and 1.98 g D(+)-Glucose monohydrate (10 mM) in Aqua sterile. Adjust pH with NaOH (2 M) to 7.4 and sterile filter the medium. Store Powell medium at 4 °C.
  3. Calcium chloride (CaCl2)
    1. Prepare a 100 mM CaCl2 solution (50 mL) and prepare aliquots containing 500 µL CaCl2. Freeze aliquots at -20 °C.
  4. Preparation of culture medium
    1. Prepare three cell culture mediums: pre-plating medium, plating medium, and washing medium. Use CCT medium as the basis for all three mediums (Table 1). Calculate CCT medium with 1 mL per culture dish. Therefore, prepare 20 mL CCT medium for 20 culture dishes (inner diameter: 35 mm).
    2. Cell culture plates: Coat each cell culture plate (inner diameter: 35 mm) with 1 mL pre-plating medium. Store the coated plates at 37 °C overnight or for at least 2 h before using.

2. Isolation of Adult Cardiomyocytes

  1. Preparation of Langendorff perfusion system
    1. Heat plating medium and washing medium to 37 °C. Defreeze a tube of 500 µL CaCl2 and weigh in 25 mg of collagenase.
    2. Flush the Langendorff perfusion system with aqua sterile, afterwards let Powell medium circulate the system for 5 min.
    3. Fill the Langendorff perfusion system with 80 mL Powell medium without any air bubbles and gas the medium with 95% oxygen.
    4. Prepare a tube (50 mL) with 40 mL Powell medium, heat it to 37 °C, and gas it with 95% oxygen.
    5. Prepare a thread of about 25 cm in length for attaching the removed heart to the cannula.
    6. Degrease a razor blade with alcohol (70% by volume) and fasten it to the chopper. Clamp a plastic disc into the chopper.
  2. Extraction of heart
    1. Anesthetize a male wistar rat with 4% to 5% isoflurane and sacrifice it with cervical dislocation. Open the abdomen behind the costal arch with an abdominal shear and, with the same pair of scissors, cut through the diaphragm to open the thoracic cavity.
    2. Remove the heart, together with the lung and thymus, by cutting above the thymus highly cranial in the thoracic cavity. Transfer the material to ice-cold saline solution immediately.
    3. Remove the lung and thymus from the heart with a dissecting scissor (large) and by fixating the material with capsule forceps, transfer the latter to a new saline solution.
  3. Isolation
    1. Remove excess tissue, like residues of thymus, trachea, fat, and connective tissue from the heart using capsule forceps and a dissecting scissor (large or small). Uncover the aorta and sever it with a dissecting scissor (large or small) between the first and second branchial arch.
    2. Start the dripping of the Langendorff perfusion system. Place the heart on the cannula of the Langendorff perfusion system and fixate it first with a crocodile clamp and later with the prepared thread. Rinse the heart until it is free of blood.
    3. Dissolve 25 mg Collagenase in 5 - 6 mL warm Powell medium and add 12.5 µL CaCl2 (30 µM).
    4. Close circulation by moving a glass funnel, which is connected with the Langendorff perfusion system, over the dripping heart and add the solved collagenase to the perfusion system. Start the perfusion for 25 min with a drop velocity of 1 drop per second.
      NOTE: During perfusion, the heart will swell and get a waxy appearance.
    5. Stop the perfusion after 25 min and remove the heart from the Langendorff perfusion system. Remove the aorta, atria, and connective tissue from the heart and open the right and left ventricles.
    6. Chop the heart two times at an angle of 90° (cutting width: 0.7 mm; velocity: 0.15 cm/s). Repeat this process manually with two scalpels for 10 s each side.
    7. Transfer 12 mL of the perfusion medium into a new tube (50 mL). Pour the cell slurry into this medium and digest cells for another five minutes at 37 °C. Mix the solution every minute.
    8. Filter the solution with the digested heart through a nylon mesh (200 µm) into a new tube (50 mL).
    9. Centrifuge the filtered solution at 29 x g for 3 min. Discard the supernatant and add 6 mL warm Powell medium including 12.5 µL CaCl2 (250 µM) to the cell pellet Resuspend the pellet through smooth shaking movements. Centrifuge again at 29 x g for 2 min. Discard the supernatant and add 6 mL warm Powell medium substituted with 25 µL CaCl2 (500 µM). Dissolve the cell pellet through gentle shaking movements and add 12 mL warm Powell medium including 120 µL CaCl2 (1 mM). Centrifuge for a third time at 16 x g for 1 min. Again, remove the supernatant.
    10. Mix the cell pellet with the pre-warmed plating medium.
    11. Remove pre-plating medium from culture plates. Transfer 1 mL plating medium, including the isolated cardiomyocytes, to each culture plate. Incubate fresh isolated cardiomyocytes at 37 °C for 1 h.
    12. Remove the plating medium from culture plates. Add 1 mL washing medium to each culture plate and store the plates at 37 °C up to six days without changing the medium.
    13. For investigating the influence of different chemicals and treatments on ARVC, first refresh plating medium by washing medium and thereafter adding different chemicals.
      NOTE: Evaluation with light microscopy: With each cell preparation, 150 to 300 cardiomyocytes should be monitored per day by light microscopy Subdivide all counted cardiomyocytes into groups according to their appearance (e.g., "rod-shaped", "round down", "spreading", and "unusual appearance"). The category "spreading" includes all cardiomyocytes with pseudopodia-like structures. "Unusual appearance" includes all ARVC with an irregular surface and no detectable intact cell membrane.

3. Example Experiments

  1. Fluorescence/Immunofluorescence staining of adult cardiomyocytes
    1. Analyze morphological and structural conversions of ARVC during cultivation by confocal laser microscopy. Use Phalloidin-TRITC to investigate F-actin structures in "rod-shaped", "round down", and "spreading" ARVC. Perform staining according to the manufacturer's protocol. An example for Phalloidin-TRITC staining is given in reference Nippert et al.11. With fluorescence/immunofluorescence staining, differences in the de- and re-differentiation of the contractile apparatus in cultivated adult cardiomyocytes between experimental treatments (e.g., with Swiprosin-1, ionomycin) can be investigated.
  2. Real-time quantitative RT-PCR (qRT-PCR)
    1. Perform qRT-PCR to investigate changes in the mRNA expression of different genes (e.g., OSM, Swiprosin-1, β-MHC) during cultivation of ARVC. For sufficient sample size, use larger culture dishes (inner diameter: 60 mm) with 2 mL volume. ARVC of five culture dishes yields one sample. Perform isolation of mRNA and transformation of cDNA according to the manufacturer's protocol.
  3. Immunoblot techniques
    1. Perform Western Blots to investigate changes in protein expression (e.g., for Swiprosin-1) during cultivation of ARVC. Use one culture dish (1 mL) per sample.

Subscription Required. Please recommend JoVE to your librarian.

Representative Results

Adult cardiomyocytes in culture: Figure 1 shows an overview of freshly isolated adult cardiomyocytes 2 h after the last washing. Approximately 75% of all cardiomyocytes had a rod-shaped morphology. The remaining 25% showed an unusual appearance with a round morphology and no detectable intact cell membrane (Figure 1). At the end of cultivation (day 6), up to 15% of all cardiomyocytes showed spreading, about 10% remained in a round morphology without pseudopodia-like structures, and 75% of all cardiomyocytes presented an unusual appearance with an irregular surface and without a detectable intact cell membrane (data not shown).

Figure 1
Figure 1: Overview of freshly isolated rat cardiomyocytes. The fraction of freshly isolated cardiomyocytes which showed a rod-shaped morphology amounted to 75% of cardiomyocytes, on average. The remaining 25% of cells presented an unusual appearance with an irregular surface and no detectable intact cell membrane. Recording was conducted by light microscopy 2 h after washing the freshly isolated cardiomyocytes. Light microscopy 2X magnification Please click here to view a larger version of this figure.

With light microscopy, freshly isolated ARVC appeared rod shaped and around 100 µm in size (Figure 2A). Freshly isolated ARVC that contract spontaneously were not calcium tolerant. All cells that were round and without a detectable intact cell membrane were damaged and not viable (Figure 2A-B). In the following days, most of the rod shaped ARVC lost this morphology. Cells got rounded with a detectable intact cell membrane. These ARVC were viable. Starting at day three the latter cells formed pseudopodia-like structures. Some of these ARVC kept their rounded appearance during spreading (Figure 2B). Others converted into flat, polymorphic ARVC (Figure 2B).

Figure 2
Figure 2: Isolated rat cardiomyocytes. (A) Freshly isolated ARVC were typically rod-shaped. (B) After six days in culture, pseudopodia-like structures (spreading) were clearly detectable in the now rounded ARVC. Some ARVC completely changed to a widespread morphology. ARVC with an unusual appearance displayed an irregular surface and no detectable intact cell membrane. Light microscopy 10X magnification. Please click here to view a larger version of this figure.

Freshly isolated ARVC were typically rod shaped with a clearly visible cross striation (Figure 3, Day 0). Changes in cell morphology were observed during the following days in culture. First, ARVC lost all their contractile elements (Figure 3, Days 1 and 2). This was followed by a reformation, implicating de novo sarcomerogenesis. The reformation was preceded by the formation of pseudopodia-like structures (spreading, Figure 3, Days 3 to 6). De novo sarcomerogenesis started with the appearance of actin stress fibers (Figure 3, Day 3). Additionally, actin bundles appeared in the perinuclear region and formed newly assembled sarcomeres (Figure 3, Days 4 and 5). The latter grew along the preformed actin stress fibers into the periphery (Figure 3, Day 6). At the end of the cultivation period (Day 6a), a typical cross striation from newly assembled sarcomeres in the spread ARVC was observed.

Figure 3
Figure 3: Fluorescence staining. The de- and re-differentiation of ARVC in culture with 20% FCS is shown. Freshly isolated ARVC with their typical rod shape (Day 0) became round by degrading sarcomeres during the first days of culture (Day 1). They lost all their contractile elements (Day 2) followed by formation of pseudopodia-like structures (spreading; Days 3 - 5) and subsequent reformation of their contractile elements indicating de novo sarcomerogenesis (Day 6). At day six in culture, cross striation was clearly detectable again (Day 6a). Staining with Phalloidin-TRITC according to the manufacturer's protocol; "arrows": pseudopodia-like structures (example shown); *: actin bundles in the perinuclear region (exemplary shown). Parts of this figure are published in11. Please click here to view a larger version of this figure.

Figure 4 displays the kinetic of the spreading process during cultivation. The fraction of ARVC showing pseudopodia-like structures at each time of examination is given as spreading in % (Figure 4). Spreading started around day three and increased constantly during the time of cultivation. 14.7% ± 1.39% of all counted ARVC showed pseudopodia-like structures after six days in cultivation.

Figure 4
Figure 4: Spreading kinetic increase in cardiomyocytes with pseudopodia-like structures normalized to all counted cardiomyocytes (spreading in %) during six days of cultivation time (n = 33 cell preparations). Data are presented as means ± SEM. This figure is published in11. Please click here to view a larger version of this figure.

Effect of ionomycin on the spreading of ARVC: The isolation and cultivation of ARVC is a calcium sensitive process1,8. Treatment of ARVC with ionomycin (1 µM), which increases intracellular calcium concentration, caused a significant (p ≤0.01) decrease in the formation of pseudopodia-like structures compared to controls (Figure 5). When compared directly, 17.19% ± 2.45% of all counted ARVC showed spreading under control conditions but only 9.87% ± 2.77% of all counted ARVC formed pseudopodia-like structures in the presence of ionomycin (day 6 of cultivation). Thus, ionomycin reduced spreading by 42.58%.

Figure 5
Figure 5: Spreading kinetics under treatment with ionomycin. Treatment with ionomycin (1 µM) at day 0 caused a highly significant reduction in cell spreading compared to control. Data are presented as means ± SEM; n = 4 cell preparations; Mann-Whitney-U test; * p ≤0.05; ** p ≤0.01 Please click here to view a larger version of this figure.

Additionally, ionomycin increased the percentage of ARVC with an unusual appearance compared to control conditions (Figure 6). At day six, 71.11% ± 4.65% of all counted ARVC treated with ionomycin showed an unusual appearance. However, under control conditions, only 51.35% ± 3.55% of the ARVC were categorized in this group.

Figure 6
Figure 6: ARVC with an unhealthy appearance. Treatment with ionomycin (1 µM) at day 0 caused a significant increase in the number of ARVC, which showed an unusual appearance. At day 6, the difference between control and ARVC treated with ionomycin was significant. Data are presented as means ± SEM; n = 4 cell preparations; Mann-Whitney-U test; * p ≤0.05 Please click here to view a larger version of this figure.

At day 3 of cultivation, under treatment with ionomycin, qRT-PCR revealed a decrease in mRNA expression of β-MHC (p ≤0.01) and OSM, which both play a distinct role in the de-differentiation of ARVC (Figure 7A and C). Swiprosin-1, a marker for re-differentiation of ARVC was significantly downregulated, too (Figure 7B).

Figure 7
Figure 7: De- and re-differentiation of cultivated ARVC under treatment with ionomycin
(1 µM) at day 0 caused a decreased mRNA expression of oncostatin M (OSM) and β-MHC, which both play key roles in the de-differentiation of adult cardiomyocytes. Additionally, mRNA expression of Swiprosin-1, a key player in the re-differentiation of adult cardiomyocytes, was also decreased by ionomycin treatment. Day 3 of cultivation; Data are presented as means ± SEM; n = 30 cell culture plates per group; Mann-Whitney-U test; * p ≤0.05; ** p ≤0.01 Please click here to view a larger version of this figure.

Pre-plating medium
20 mL CCT medium
2 % Vol. Penicillin/Streptomycin (400 μL)
4 % Vol. FCS (800 μL)
Plating medium
20 mL CCT medium
2 % Vol. Penicillin/Streptomycin (400 μL)
Washing medium
20 mL CCT medium
2 % Vol. Penicillin/Streptomycin (400 μL)
Note: 4 %Vol. FCS in pre-plating medium can be replaced by 1 Vol.-% laminin (0.5 μg/cm2). Additionally, for cultivating cardiomyocytes for several days add 20 Vol.-% FCS to the washing medium. Store plating medium and washing medium by 4-8 °C until using.

Table 1: Culture media used for cardiomyocyte isolation

Subscription Required. Please recommend JoVE to your librarian.

Discussion

The behavior of adult cardiomyocytes in vivo is influenced by many interactions with other cells (e.g., neurons, endothelial cells, fibroblasts, inflammatory cells) and the electrical syncytium which they form1. Therefore, studying stress adaptation of adult cardiomyocytes exclusively requires the isolation and cultivation of ARVC. The main effects of isolating and cultivating ARVC are: 1) disconnecting them from extracellular matrix and cell-cell contacts; 2) disconnecting them from contractile stimuli; 3) forcing them to adapt from a three-dimensional tissue to two-dimensional surroundings. Under these conditions, ARVC start de- and re-differentiation as described above and perform multiple adaptations, which are also seen during cardiac remodeling in vivo (β-adrenoceptor desensitization, reassembly of sarcomeres, etc.)4. Therefore, the isolation of adult cardiomyocytes represents a valid method to investigate these cells and their response to different treatments. These insights can be used afterwards for in vivo experiments, which would aid in avoiding unnecessary experiments and reducing the number of test animals. Certainly, some findings seen in vitro will not occur in vivo (e.g., the formation of pseudopodia-like structures). The existing cell-cell-contacts within the electrical syncytium will hamper excessive growth under physiological conditions17. Nevertheless, isolated and cultivated ARVC can be used to investigate the behavior of adult cardiomyocytes. Additionally, first trials of new treatment strategies against cardiac diseases in humans can be conducted with ARVC.

The described method for the isolation and cultivation of adult cardiomyocytes contains some critical points. To obtain successful results, the following items have to be considered.

1. Calcium tolerance: Historically, the calcium tolerance of adult cardiomyocytes was one of the most critical factors leading to a successful isolation and cultivation of adult cardiomyocytes1,7,8. Nowadays, protocols are established to ensure cultivation under physiological calcium conditions1,3. This manuscript shows the influence of a changed intracellular calcium concentration on the quality of isolated ARVC. Ionomycin, which increases intracellular calcium concentrations, caused a significant decrease in spreading and a significant increase in the number of cardiomyocytes with an unusual appearance. Furthermore, it caused a downregulation of the key markers for cardiac de- and re-differentiation: β-MHC, OSM, and Swiprosin-1. Therefore, changing the intracellular calcium concentration during cultivation hampers the capability of ARVC to adapt to new environments. Although some ARVC were able to spread and adapt (9.87% ± 2.77% of all counted ARVC; Figure 5), an accurate investigation of ARVC under these conditions is not possible. Consequently, for a precise isolation and cultivation of ARVC an established calcium protocol should be used. Additionally, it should be ensured that none of the investigated treatments interfere with the calcium hemostasis of ARVC.

2. Collagenase: There are different batches of collagenase available. Each batch shows differences in quality and effectiveness1. Therefore, the authors recommend ordering and testing samples of different batches. Additionally, the time of digestion and the amount of collagenase of each new batch used needs to be evaluated separately. Accordingly, the concentration and time of digestion in the protocol described can differ slightly to other protocols.

3. Time until heart perfusion: To ensure a high quality of ARVC, the time between extracting the heart from the body and the start of perfusion with the Langendorff system should be as short as possible. A prolonged time causes damage to the heart and results in a higher number of non-viable ARVC.

Additionally, warming the perfusion solution during the perfusion and digestion for 5 minutes after chopping the tissue is essential to yielding a good result. To avoid unnecessary damage of the biological tissue, it should be handled carefully at all-time points. Furthermore, it should be pointed out that treatment with OSM or lower concentrations of FCS also enable ARVC to de- and re-differentiate4,10,18,19. However, without these nutritive treatments, ARVC degenerate within a few days2.

In conclusion, the isolation and cultivation of ARVC is a sensitive method that offers a variety of possibilities to investigate the behavior of adult cardiomyocytes exclusively.

Subscription Required. Please recommend JoVE to your librarian.

Disclosures

The results shown are part of the doctoral thesis of Franziska Nippert.

Acknowledgements

The authors thank Nadine Woitasky and Peter Volk for technical assistance. Additionally, the authors thank Mrs. Claudia Lorenz (medical writer, ACCEDIS) for her help in preparing the manuscript. This manuscript was financially supported by DFG (Schlu 324/7-1).

Materials

Name Company Catalog Number Comments
Langendorff perfusion system inhouse construction double-walled with a water
based heating system
Tissue chopper Mc Ilwain Cavey Laboratory Engeneering Co. Ltd.
Aortic Cannula, OD 1,8 mm inhouse construction
Abdominal shears Aeskulap BC772R
Capsule forceps Eickemeyer 171307
Dissecting scissor large Aeskulap BC562R
Dissecting scissor small Aeskulap BC163R
Mash with Polyamid Neolab 4-1413 mash size 200 μm
plastic disc Cavey Laboratory Engeneering Co. Ltd.
Collagenase Typ II Worthington LS004177

DOWNLOAD MATERIALS LIST

References

  1. Schlüter, K. -D. Cardiomyocytes - Active Players in Cardiac Disease. Springer International Publishing AG Switzerland. (2016).
  2. Piper, H. M., Probst, I., Schwartz, P., Hutter, F. J., Spieckermann, P. G. Culturing of calcium stable adult cardiac myocytes. J. Mol. Cell. Cardiol. 14, (7), 397-412 (1982).
  3. Schlüter, K. -D., Schreiber, D. Adult ventricular cardiomyocytes: isolation and culture. Protein Tyrosine Kinases: From Inhibitors to Useful Drugs. Fabbro, D., McCormick, F. 290, Humana Press Inc. Totowa, NJ. 305-314 (2005).
  4. Eppenberger, M. E., et al. Immunocytochemical analysis of the regeneration of myofibrils in long-term cultures of adult cardiomyocytes of the rat. Dev. Biol. 130, (1), 1-15 (1988).
  5. Burrows, M. T. The cultivation of tissues of the chick-embryo outside the body. JAMA. 55, (24), 2057-2058 (1910).
  6. Cavanaugh, M. W. Pulsation, migration and division in dissociated chick embryo heart cells in vitro. J Exp Zool A Eco Genet Physiol. 128, (3), 573-589 (1955).
  7. Muir, A. R. Further observation on the cellular structure of cardiac muscle. J. Anat. 99, 27-46 (1965).
  8. Powell, T., Twist, V. W. A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and a tolerance to calcium. Biochem. Biophys. Res. Com. 72, (1), 327-333 (1976).
  9. Schwarzfeld, T. Isolation and development in cell culture of myocardial cells of the adult rat. J. Mol. Cell. Cardiol. 13, (6), 563-575 (1981).
  10. Kubin, T., et al. Oncostatin M is a major mediator of cardiomyocyte dedifferentiation and remodeling. Cell Stem Cell. 9, (5), 420-432 (2011).
  11. Nippert, F., Schreckenberg, R., Hess, A., Weber, M., Schlüter, K. -D. The effects of Swiprosin-1 on the formation of pseudopodia-like structures and β-adrenoceptor coupling in cultured adult rat ventricular cardiomyocytes. PLoS ONE. 11, (12), e0167655 (2016).
  12. Moses, R. L., Claycomb, W. C. Disorganization and reestablishment of cardiac muscle cell ultrastructure in cultured adult rat ventricular muscle cells. J. Ultrastruct. Res. 81, (3), 358-374 (1982).
  13. Eppenberger-Eberhardt, M., Flamme, I., Kurer, V., Eppenberger, H. M. Reexpression of α-smooth muscle actin isoform in cultured adult rat cardiomyocytes. Dev. Biol. 139, (2), 269-278 (1990).
  14. Fedak, P. W., Verma, S., Weisel, R. D., Skrtic, M., Li, R. K. Cardiac remodeling and failure: from molecules to man (Part III). Cardiovasc. Pathol. 14, (3), 109-119 (2005).
  15. Leri, A., Kajstura, J., Anversa, P. Mechanisms of myocardial regeneration. Trends Cardiovasc. Med. 21, (2), 52-58 (2011).
  16. Cohn, J. N., Ferrari, R., Sharpe, N. Cardiac remodeling-concepts and clinical implications: A consensus paper from an international forum on cardiac remodeling. J. Am. Coll. Cardiol. 35, (3), 569-582 (2000).
  17. Alberts, B., et al. Molecular biology of the cell. Garland Science Taylor and Francis Group. New York, NY. (2015).
  18. Pöling, J., et al. The Janus face of OSM-mediated cardiomyocyte dedifferentiation during cardiac repair and disease. Cell Cycle. 11, (3), 439-445 (2014).
  19. Decker, M. L., Behnke-Barclay, M., Cook, M. G., Lesch, M., Decker, R. S. Morphometric evaluation of the contractile apparatus in primary cultures of rabbit cardiac myocytes. Circ. Res. 69, (1), 86-94 (1991).

Comments

0 Comments


    Post a Question / Comment / Request

    You must be signed in to post a comment. Please or create an account.

    Video Stats