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Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the Neurotransmitter Glutamate In Vitro
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Bioengineering
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JoVE Journal Bioengineering
Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the Neurotransmitter Glutamate In Vitro

Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the Neurotransmitter Glutamate In Vitro

DOI:
10.3791/56645-v

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12:56 min

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December 19, 2017

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, ,
1Department of Mechanical and Industrial Engineering,University of Illinois at Chicago, 2Department of Biomedical Engineering,Northwestern University

Chapters

  • 00:05Title
  • 01:16Wholemount Retinal Preparation
  • 03:12MEA and Data Acquisition Setup
  • 05:23Glutamate Stimulation Preparation
  • 08:38Retinal Recording and Stimulus Program
  • 10:30Results: Electrode Recordings of Neuronal Activity During Visual, Spontaneous, and Glutamate Injection Recordings
  • 12:06Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a novel method for investigating a form of chemical neurostimulation of wholemount rat retinas in vitro with the neurotransmitter glutamate. Chemical neurostimulation is a promising alternative to the conventional electrical neurostimulation of retinal neurons for treating irreversible blindness caused by photoreceptor degenerative diseases.

Tags

BiomimeticChemical NeuromodulationRat RetinasNeurotransmitterGlutamateIn VitroPhotoreceptor DegenerationRetinal NeuronsArtificial VisionEnucleated EyesAmes’ MediumCorneaLensVitreous HumourScleraRetinaOptic NerveNylon MeshMultielectrode ArrayPMEA SurfacePerfusion

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