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Journal / Developmental Biology / 人诱导多能干细胞的前脑型脑 Organoids…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
人诱导多能干细胞的前脑型脑 Organoids 标准化和可再生性的生成
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
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Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
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Journal (Developmental Biology)
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人诱导多能干细胞的前脑型脑 Organoids 标准化和可再生性的生成

Article DOI: 10.3791/56768-v • 10:25 min • January 23rd, 2018
January 23rd, 2018 •
*1, *1, 1, 1,2,3, 1
1Institute of Reconstructive Neurobiology, University of Bonn, 2Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)
* These authors contributed equally

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脑 organoids 代表了一个新的模型系统来研究早期人脑发育体外。本文提供了详细的方法, 以有效地产生同种背前脑型 organoids 的人诱导多能干细胞, 包括关键的表征和验证步骤。

Tags

发育生物学 问题 131 诱导多能干细胞 神经生物学 脑 organoids 人皮质发育 皮质畸形 疾病模型
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