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Journal / Developmental Biology / Generasjon av standardiserte og reproduserbar…
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Developmental Biology

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JoVE Journal Developmental Biology
Generasjon av standardiserte og reproduserbar Forebrain-type Cerebral Organoids fra menneske indusert Pluripotent stamceller
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Generasjon av standardiserte og reproduserbar Forebrain-type Cerebral Organoids fra menneske indusert Pluripotent stamceller

Article DOI: 10.3791/56768-v • 10:25 min • January 23rd, 2018
January 23rd, 2018 •
*1, *1, 1, 1,2,3, 1
1Institute of Reconstructive Neurobiology, University of Bonn, 2Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)
* These authors contributed equally

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Cerebral organoids representerer en ny modellsystem å undersøke tidlig menneskelige hjerne utviklingen i vitro. Denne artikkelen gir detaljert metoder for å effektivt generere homogen rygg forebrain-type organoids fra menneskelige indusert pluripotent stamceller inkludert avgjørende karakterisering og godkjenningen skritt.

Tags

Utviklingspsykologi biologi problemet 131 utførte pluripotent stamceller nevrobiologi cerebral organoids menneskelig kortikale utvikling kortikale misdannelser sykdom modellering
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