Journal
/
Behavior
/
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
JoVE Journal
Behavior
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Behavior
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

DOI:
10.3791/56900-v

•

07:24 min

•

April 24, 2018

•

, ,
1School of Life Science,University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences

Chapters

  • 00:05Title
  • 00:51Preparing the Feeding Chamber
  • 02:58Thermogenetic Activation
  • 03:44Optogenetic Activation
  • 04:48Colorimetric Quantification of Food Consumption
  • 06:08Results: Activated Taotie-GAL4 Neurons Increase Food Intake
  • 06:55Conclusion

Summary

Automatic Translation

Automatic Translation

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

Tags

Food IntakeDrosophilaQuantitative AssayThermogenetic ActivationOptogenetic ActivationHungerSatietyAppetiteAgarose PadSucroseBlue DyeFeeding ChamberTemperature

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference

A Procedure to Study the Effect of Prolonged Food Restriction on Heroin Seeking in Abstinent Rats

Uncovering Beat Deafness: Detecting Rhythm Disorders with Synchronized Finger Tapping and Perceptual Timing Tasks

Design and Implementation of an fMRI Study Examining Thought Suppression in Young Women with, and At-risk, for Depression

Combining Multiple Data Acquisition Systems to Study Corticospinal Output and Multi-segment Biomechanics

Feeding Experimentation Device (FED): Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents

Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila

Reversible Cooling-induced Deactivations to Study Cortical Contributions to Obstacle Memory in the Walking Cat

An Automated Method to Determine the Performance of Drosophila in Response to Temperature Changes in Space and Time

In Vivo Protocol of Controlled Subconcussive Head Impacts for the Validation of Field Study Data

Read Article