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Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
JoVE Journal
Behavior
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JoVE Journal Behavior
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
DOI:

07:24 min

April 24, 2018

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Chapters

  • 00:05Title
  • 00:51Preparing the Feeding Chamber
  • 02:58Thermogenetic Activation
  • 03:44Optogenetic Activation
  • 04:48Colorimetric Quantification of Food Consumption
  • 06:08Results: Activated Taotie-GAL4 Neurons Increase Food Intake
  • 06:55Conclusion

Summary

Automatic Translation

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

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