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JoVE Journal Neuroscience
Forbereder frisk Retinal skiver fra voksen zebrafisk for Ex Vivo billeddannelse eksperimenter
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Forbereder frisk Retinal skiver fra voksen zebrafisk for Ex Vivo billeddannelse eksperimenter

Article DOI: 10.3791/56977-v • 11:01 min • May 9th, 2018
May 9th, 2018 •
1, 1, 1,2, 1,2
1Department of Biochemistry, University of Washington, 2Department of Ophthalmology, University of Washington

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Imaging retinale væv kan give én celle oplysninger, der ikke kan blive indsamlet fra traditionelle biokemiske metoder. Denne protokol beskriver forberedelse af retinale skiver fra zebrafisk for Konfokal billeddannelse. Fluorescerende kodet genetisk sensorer eller indikator farvestoffer tillade visualisering af mange biologiske processer i forskellige retinal celletyper.

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Neurovidenskab spørgsmålet 135 zebrafisk nethinden fotoreceptor væv skive fluorescens biosensor live imaging mikroskopi neurovidenskab Molekylærbiologi cellebiologi
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