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Journal / Immunology and Infection / Replikering af bestilt, Nonredundant bibliotek af …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Replikering af bestilt, Nonredundant bibliotek af Pseudomonas aeruginosa stamme PA14 Transposon indsættelse mutanter
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Replikering af bestilt, Nonredundant bibliotek af Pseudomonas aeruginosa stamme PA14 Transposon indsættelse mutanter

Article DOI: 10.3791/57298-v • 11:35 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 1, 1, 2,3, 1,4, 1,4
1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School
* These authors contributed equally

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Pseudomonas aeruginosa infektion forårsager betydelig sygelighed i sårbare værter. Nonredundant transposon indsættelse mutant bibliotek af P. aeruginosa stamme PA14, udpeget som PA14NR sæt, letter analyse af genet funktionalitet i mange processer. Præsenteres her er en protokol til at skabe høj kvalitet kopier af PA14NR indstillet mutant biblioteket.

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Immunologi og infektion spørgsmålet 135 Pseudomonas aeruginosa mariner transposon mutant bibliotek mutagenese mikrobiologi opportunistiske infektioner immunologi
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