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Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology
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Chapters
- 00:04Title
- 00:54Surgical Preparations
- 02:28Murine Nephrectomy
- 04:05Murine Kidney Microdissection for Renal Segment Isolation
- 07:00Results: Characterizing the Renal Segments
- 08:10Conclusion
Tissue-engineered renal constructs provide a solution for the organ shortage and deleterious effects of dialysis. Here, we describe a protocol to micro dissect murine kidneys for isolation of cortico-medullary segments. These segments are implanted into scaffold-free cellular constructs, forming renal organoids.
