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Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
JoVE Journal
Medicine
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JoVE Journal Medicine
Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
DOI:

09:03 min

June 19, 2018

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Chapters

  • 00:05Title
  • 01:03OT-I T Cell Staining and Adoptive Transfer
  • 04:22Endo-Free OVA +/- Adjuvant Immunization and Lymphocyte Isolation
  • 05:21Flow Cytometry
  • 07:30Results: Representative Adjuvant Drive Cytotoxic T Cell Generation
  • 08:31Conclusion

Summary

Automatic Translation

We present here an application for a standard immunological technique (CFSE stained OT-I proliferation) intended to rapidly monitor adjuvant-mediated cytotoxic T lymphocyte (CTL) generation in vivo. This fast estimation of CTL capacities is useful for the development of prophylactic vaccines against intracellular pathogens as well as therapeutic cancer vaccines.

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