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Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
DOI:

07:37 min

April 30, 2018

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Chapters

  • 00:05Title
  • 00:42Collection of Decidual Tissue from Term Placental Membranes
  • 01:57Washing and Enzymatic Digestion of the Decidual Tissue
  • 03:56Generation of Single Cell Suspension
  • 05:22Results: Use of Flow Cytometry and Immunocytochemistry to Isolate the Primary Human Decidual Cells
  • 07:11Conclusion

Summary

Automatic Translation

This protocol demonstrates a method for the isolation of primary human decidual cells collected from the fetal membranes of term placentae which can be used for a variety of applications (i.e. immunocytochemistry, flow cytometry, etc.) aiming to study the role of different cell populations in pregnancy complications.

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