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Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming Cells
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Chapters
- 00:04Title
- 00:36Preparation of Aluminum (Al) Substrate by Electropolishing
- 01:34Fabrication of Gradient Anodic Aluminum Oxide (AAO) Mold with Phosphoric Acid Electrolyte
- 04:28Deposition of Anti-Sticking Layer on Gradient AAO Mold with Self-Assembled Monolayer
- 05:46Fabrication of Gradient Nanopattern Plates by Thermal Imprinting
- 07:08Cultivation of hECFCs and Culture on the Gradient Nanopattern Plates
- 09:14Results: Evaluation and Analysis of Culturing hECFCs on Fabricated Gradient Nanopattern Plates
- 10:37Conclusion
Here, we present a protocol for the fabrication of gradient nanopattern plates via thermal nanoimprinting and the method of screening responses of human endothelial progenitor cells to the nanostructures. By using the described technology, it is possible to produce a scaffold that can manipulate cell behavior by physical stimuli.
