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In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

DOI:
10.3791/57741-v

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06:35 min

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June 15, 2018

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, , , ,
1Department of Neurosciences, Experimental Neurology,KU Leuven – University of Leuven, 2Center for Brain & Disease Research, Laboratory of Neurobiology,VIB, 3Department of Neurology,University Hospitals Leuven

Chapters

  • 00:04Title
  • 00:49Measurement of CMAP in Hind- and Forelimbs
  • 02:59Data Acquisition
  • 04:54Results: CMAP and Required Stimulus Over the Disease Course in CMT1A Mice
  • 05:50Conclusion

Summary

Automatic Translation

Automatic Translation

The measurement of nerve conduction is a useful tool to assess mouse models of neurodegeneration but it is frequently only applied to stimulate the sciatic nerve in hindlimbs. Here, we describe a technique to measure compound muscle action potential (CMAP) in vivo in the mouse forelimb muscles innervated by the brachial plexus.

Tags

In Vivo ElectrophysiologyCompound Muscle Action PotentialMouse ModelsMotor Neuron DegenerationAxon DegenerationDemyelinationPeripheral NervesMinimally InvasiveRepeated MeasurementsSensitivityEarly DetectionAnesthesiaBody Temperature RegulationElectrode PlacementForelimbHind LimbSciatic NerveGastrocnemius MuscleBrachial PlexusBiceps Brachii

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