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Immunology and Infection
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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

DOI:
10.3791/57941-v

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09:12 min

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October 17, 2018

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, , , , ,
1Department of Surgery, Vascular Biology Research Centre,Westmead Hospital, 2Westmead Clinical School, Department of Surgery,The University of Sydney, 3Westmead Research Hub,Westmead Institute for Medical Research, 4Institute for Clinical Pathology and Medical Research,Westmead Hospital

Chapters

  • 00:04Title
  • 00:42Sample Preparation for Whole Blood Flow Cytometry
  • 02:14Flow Cytometry
  • 03:28Monocyte Gating
  • 07:11Results: Validation of Gating and Quantification of Marker Expression
  • 08:38Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for characterizing monocyte subsets by whole blood flow cytometry. This includes outlining how to gate the subsets and assess their expression of surface markers and giving an example of the assessment of the expression of M1 (inflammatory) and M2 markers (anti-inflammatory).

Tags

Monocyte SubsetsFlow CytometryWhole Blood AnalysisCD14CD16HLA-DRM1 And M2 MarkersGatingRed Blood Cell LysisWhite Blood Cell FixationSample PreparationFlow Cytometry SetupQuality Control

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