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Journal / Immunology and Infection / In Vitro Canine neutrofiele extracellulaire Trap…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Canine neutrofiele extracellulaire Trap vorming
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In Vitro Canine neutrofiele extracellulaire Trap vorming: Dynamische en kwantitatieve analyse door fluorescentie microscopie

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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We beschrijven de methoden om te isoleren canine neutrofielen van volbloed en visualiseren van netto vorming in levende neutrofiele granulocyten met behulp van fluorescentie microscopie. Ook beschreven zijn protocollen te kwantificeren netto vorming en citrullinated Histon H3 (citH3)-expressie met immunofluorescentie microscopie.

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Immunologie en infecties probleem 138 immunofluorescentie microscopie sepsis granulocyt isolatie Histon Citrullinatie ontstaan deiminase
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