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Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
DOI:

05:08 min

August 24, 2018

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Chapters

  • 00:04Title
  • 00:27Large Scale Culture on Plates
  • 01:15Transfer of Large Amounts of Caenorhabditis elegans
  • 01:52Harvest of a Large Caenorhabditis elegans Sample
  • 03:20Protein Isolation for Mass Spectrometry
  • 03:56Results: Reproducible Quantitative Protein Isolation and Further Applications of Large-scale C. elegans Culture
  • 04:47Conclusion

Summary

Automatic Translation

This protocol describes a method for the large-scale cultivation of Caenorhabditis elegans on solid media. As an alternative to liquid culture, this protocol allows obtaining parameters of different scales under plate-based cultivation. This increases the comparability of results by omitting the morphological and metabolic differences between liquid and solid media culture.

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