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Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
DOI:

08:04 min

October 23, 2018

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Chapters

  • 00:04Title
  • 00:47Liver Perfusion and Extraction
  • 02:45Primary Mouse Hepatocyte Isolation
  • 04:07Polyacrylamide Gel Electrophoresis (PAGE) Analysis
  • 06:06Results: Representative Nascent Protein Expression Analyses
  • 07:31Conclusion

Summary

Automatic Translation

Here, we present a protocol for the isolation of healthy and functional primary mouse hepatocytes. Instructions for detecting hepatic nascent protein synthesis by non-radioactive labeling substrate were provided to help understand the mechanisms underlying protein synthesis in the context of energy-metabolism homeostasis in the liver.

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