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JoVE Journal
Immunology and Infection

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成年斑马鱼的口服插管
 
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成年斑马鱼的口服插管: 一种评价生物活性化合物肠道吸收的模型

Article DOI: 10.3791/58366-v 04:33 min September 27th, 2018
September 27th, 2018

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Summary

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Click here for the English version.

该协议描述插管成年斑马鱼与生物;然后对肠道进行解剖和制备, 用于细胞术、共焦显微镜和 qPCR。这种方法允许管理生物活性化合物, 以监测肠道摄取和局部免疫刺激诱发。对口腔预防手段的肠道动态检测有重要意义。

Tags

免疫学和感染 问题 139 斑马鱼 口服插管 肠道 黏膜免疫 细胞术 共焦成像 qPCR
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