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Journal / Bioengineering / Gen Expression analyse i Endothelial celler udsat …
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JoVE Journal Bioengineering
Gen Expression analyse i Endothelial celler udsat for Shear Stress ved hjælp af flere Parallel-plade Flow kamre
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Gen Expression analyse i Endothelial celler udsat for Shear Stress ved hjælp af flere Parallel-plade Flow kamre

Article DOI: 10.3791/58478-v • 08:50 min • October 21st, 2018
October 21st, 2018 •
1,2, 1,2, 2,3, 1,2, 1,2, 1,2,3,4
1Institute of Medical Science, University of Toronto, 2Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Department of Medicine, University of Toronto

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Her præsenteres en arbejdsproces for kultur og gen expression analysen i endothelial celler under væske shear stress. Inkluderet er en fysisk arrangement for samtidig boliger og overvågning flere flow kamre i et kontrolleret miljø og brugen af en udefrakommende reference RNA til kvantitativ PCR.

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Bioteknologi spørgsmål 140 Flow kammer shear stress laminar flow parallel-plade overvågning endotel Gen-ekspression genregulering kvantitativ PCR normalisering luciferase reference RNA
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