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Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
JoVE Journal
Biology
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JoVE Journal Biology
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
DOI:

07:39 min

June 04, 2019

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Chapters

  • 00:04Title
  • 00:51Molecular Beacon (MB) Nurse Cell Microinjection
  • 02:38Tracking and Colocalization Analyses and Video File Preparation
  • 05:58Results: Representative MB Visualization and Tracking
  • 07:15Conclusion

Summary

Automatic Translation

Here, we present a protocol for the visualization, detection, analysis and tracking of endogenous mRNA trafficking in live Drosophila melanogaster egg chamber using molecular beacons, spinning disc confocal microscopy, and open-source analysis software.

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