Journal
/
Biology
/
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

DOI:
10.3791/58545-v

•

07:39 min

•

June 04, 2019

•

, , ,
1Biological Sciences Department Hunter College,City University of New York, 2Program in Molecular, Cellular, and Developmental Biology,Graduate Center, City University of New York

Chapters

  • 00:04Title
  • 00:51Molecular Beacon (MB) Nurse Cell Microinjection
  • 02:38Tracking and Colocalization Analyses and Video File Preparation
  • 05:58Results: Representative MB Visualization and Tracking
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the visualization, detection, analysis and tracking of endogenous mRNA trafficking in live Drosophila melanogaster egg chamber using molecular beacons, spinning disc confocal microscopy, and open-source analysis software.

Tags

MRNADrosophila MelanogasterEgg ChambersRNA LocalizationLive ImagingMolecular BeaconsMicroinjectionRNA BiologyReal-time VisualizationRNA TraffickingTherapeutic TargetsZebrafishCell CultureMicroscopyImage Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Dissection of Larval CNS in Drosophila Melanogaster

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes

Visualizing the Beating Heart in Drosophila

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Isolation of Drosophila melanogaster Testes

Preparing Individual Drosophila Egg Chambers for Live Imaging

Measurement of Lifespan in Drosophila melanogaster

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Maintenance of a Drosophila melanogaster Population Cage

Read Article