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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
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1Biosciences Laboratory,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Department of Medical Oncology,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 3Unit of Biostatistics and Clinical Trials,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS
Chapters
- 00:04Title
- 00:57Extraction of Circulating miRNAs from Plasma Samples
- 03:22Perform Reverse Transcription and miRNA Pre-amplification
- 05:28Perform Real-time PCR
- 06:05Results: Increase in hsa-miR-155-5p Associated with Shorter PFS and OS
- 07:10Conclusion
We present a protocol to evaluate the expression levels of circulating microRNA (miRNAs) in plasma samples from cancer patients. In particular, we used a commercially available kit for circulating miRNA extraction and reverse transcription. Finally, we analyzed a panel of 24 selected miRNAs using real-time pre-spotted probe custom plates.
