Journal
/
/
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
DOI:

08:12 min

March 14, 2019

, , , ,

Chapters

  • 00:04Title
  • 00:57Extraction of Circulating miRNAs from Plasma Samples
  • 03:22Perform Reverse Transcription and miRNA Pre-amplification
  • 05:28Perform Real-time PCR
  • 06:05Results: Increase in hsa-miR-155-5p Associated with Shorter PFS and OS
  • 07:10Conclusion

Summary

Automatic Translation

We present a protocol to evaluate the expression levels of circulating microRNA (miRNAs) in plasma samples from cancer patients. In particular, we used a commercially available kit for circulating miRNA extraction and reverse transcription. Finally, we analyzed a panel of 24 selected miRNAs using real-time pre-spotted probe custom plates.

Related Videos

Read Article