Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages <em>In Vitro</em> and <em>In Vivo</em>

Name: Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
Uploaded: 2019-02-05T13:00:00+00:00
Duration: 9 min 36 s
Description: Watch this Scientific Journal Video about Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo at JoVE.com

Na Jia; Haoan Wu; Juanli Duan; Chunxue Wei; Kaixuan Wang; Yu Zhang&#160;; Xiaohua Mao

doi:10.3791/58660

Journal

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Medicine

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Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

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JoVE Journal Medicine

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

DOI:

10.3791/58660-v

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09:36 min

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February 05, 2019

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Na Jia*¹, Haoan Wu*², Juanli Duan, Chunxue Wei, Kaixuan Wang, Yu Zhang , Xiaohua Mao

¹Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine,Southeast University, ²State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering,Southeast University

Chapters

00:04Title
01:00Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
03:52PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
05:00In Vitro RAW264.7 Macrophages Transfection
06:55Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
08:51Conclusion

Summary

Automatic Translation

We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

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