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Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
JoVE Journal
Medicine
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JoVE Journal Medicine
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
DOI:

09:36 min

February 05, 2019

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Chapters

  • 00:04Title
  • 01:00Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
  • 03:52PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
  • 05:00In Vitro RAW264.7 Macrophages Transfection
  • 06:55Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
  • 08:51Conclusion

Summary

Automatic Translation

We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.

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