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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
JoVE Journal
Genetics
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JoVE Journal Genetics
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
DOI:

07:10 min

February 19, 2019

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Chapters

  • 00:04Title
  • 00:38Bacterial Inoculum Preparation and Infection
  • 02:17Organ Harvest and Tissue Processing
  • 03:20Laser Scanning Confocal Microscopy and Image Processing
  • 04:52Results: Representative Localization of nuc-sGFP in the Staphylococcal Abscess Microenvironment
  • 06:22Conclusion

Summary

Automatic Translation

Described here is a method for analyzing bacterial gene expression in animal tissues at a cellular level. This method provides a resource for studying the phenotypic diversity occurring within a bacterial population in response to the tissue environment during an infection.

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