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Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
JoVE Journal
Engineering
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JoVE Journal Engineering
Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
DOI:

08:04 min

January 26, 2019

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Chapters

  • 00:04Title
  • 00:37Microtubule Preparation
  • 02:02Motility Solution
  • 03:06Assembling the Flow Cells
  • 05:08Flowing the Solutions into a Flow Cell
  • 05:45Imaging a Flow Cell
  • 06:41Results: Analysis of Assembled Molecular Shuttles
  • 07:31Conclusion

Summary

Automatic Translation

We present a protocol to build molecular shuttles, where surface-adhered kinesin motor proteins propel dye-labelled microtubules. Weak interactions of the kinesins with the surface enables their reversible attachment to it. This creates a nanoscale system which exhibits dynamic assembly and disassembly of its components while retaining its functionality.

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