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JoVE Journal Bioengineering
Såning og Implantation af en biosyntetiske væv-manipuleret trakeal Graft i en musemodel
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Såning og Implantation af en biosyntetiske væv-manipuleret trakeal Graft i en musemodel

Article DOI: 10.3791/59173-v • 09:57 min • April 1st, 2019
April 1st, 2019 •
*1,2, *1,3, 1,2, 4, 5, 3,6, 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital
* These authors contributed equally

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Transplantat stenose udgør en afgørende hindring i tissue Engineering luftvejene udskiftning. For at undersøge cellulære mekanismer bag stenose, benytter vi en murine model af væv manipuleret trakeal udskiftning med seedede knoglemarv mononukleære celler (BM-MNC). Her, detalje vi vores protokol, herunder stillads produktion, BM-MNC isolation, graft såning og implantation.

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Bioteknologi spørgsmålet 146 electrospinning syntetiske stillads medicin luftrør graft celle såning biomaterialer luftrør sygdomme
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