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Journal / Genetics / In vivo anvendelse af sen-kontrol-System for…
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Genetics

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JoVE Journal Genetics
In vivo anvendelse af sen-kontrol-System for Manipulation af endogene genekspression
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In vivo anvendelse af sen-kontrol-System for Manipulation af endogene genekspression

Article DOI: 10.3791/59235-v • 08:54 min • March 29th, 2019
March 29th, 2019 •
1, 2, 1, 1, 1, 1
1Center for Epigenetics, Van Andel Research Institute, 2Amgen

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Denne protokol beskriver de nødvendige skridt for at generere en modelsystem hvor transkription af en endogen gen af interesse betinget kan styres i levende dyr eller celler ved hjælp af forbedrede lac repressor og/eller tet aktivator systemer.

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Genetik sag 145 transskription udtryk vendbar lac repressor tet aktivator Dnmt1 undertrykkelse genregulering inducerbar
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