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Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
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Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

DOI:
10.3791/59249-v

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10:50 min

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March 26, 2019

•

,
1Theodor Kocher Institute,University of Bern

Chapters

  • 00:04Title
  • 00:53Immunization of C57BL/6 Mice
  • 02:04Scoring of EAE Mice
  • 03:08In Vivo Permeability Assay
  • 04:25Dissection and Freezing of Brains
  • 06:15In Situ Zymography Combined with Laminin/CD45 Immunofluorescence Staining
  • 08:21Results: Course of EAE and In Vivo Permeability
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present protocols to investigate impairment of the neurovascular unit during experimental autoimmune encephalomyelitis in vivo. We specifically address how to determine blood-brain barrier permeability and gelatinase activity involved in leukocyte migration across the glia limitans. 

Tags

Experimental Autoimmune EncephalomyelitisBlood-brain BarrierMatrix MetalloproteinasesImmune Cell InfiltrationNeuroinflammationEAE ScoringMouse ModelMOG PeptideFreund’s AdjuvantPertussis Toxin

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