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Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans
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Developmental Biology
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JoVE Journal Developmental Biology
Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans

DOI:
10.3791/59301-v

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09:36 min

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March 23, 2019

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, ,
1Department of Diagnostic and Biomedical Sciences, School of Dentistry,University of Texas Health Science Center

Chapters

  • 00:04Title
  • 00:29Preparation of THY (Todd-Hewitt Yeast Extract) Agar Plates and Mitis Group Streptococci
  • 02:12Survival Assays
  • 04:04Preparation of Agarose Pads for Microscopy
  • 04:52Observation of SKN-1 Localization in Response to Streptococcus Infection
  • 07:36Results: Killing of the Worms
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

The nematode Caenorhabditis elegans is an excellent model to dissect host-pathogen interactions. Described here is a protocol to infect the worm with members of the mitis group streptococci and determine activation of the oxidative stress response against H2O2 produced by this group of organisms.

Tags

Oxidative StressMitis Group StreptococciCaenorhabditis ElegansHydrogen PeroxidePathogenicityStress ResponseReactive Oxygen SpeciesTodd Hewitt AgarTHY AgarCatalaseE. Coli OP-50NGM PlatesMicroaerophilic Environment

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