00:40Culture Human PVAT Cells from the Stromal Vascular Fraction
05:04Plate and Induce Adipogenic, Osteogenic, and Chondrogenic Lineages
08:05Culture Adipogenic, Osteogenic, and Chondrogenic Lineages for 14 Days
08:20Results: Progenitor Cells from Human Aortic Perivascular Adipose Tissue of Coronary Artery Bypass Grafting Patients Exhibit Adipogenic and Chondrogenic Differentiation but Fail to Commit to Ostegenic Lineages
The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages.