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Journal / Genetics / Multi-locus Variable-número Tándel-repetir…
JoVE Journal
Genetics

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JoVE Journal Genetics
Multi-locus Variable-número Tándel-repetir Análisis de la Bacteria Patógena de Peces Yersinia ruckeri por Multiplex PCR y Electroforesis Capilar
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Multi-locus Variable-número Tándel-repetir Análisis de la Bacteria Patógena de Peces Yersinia ruckeri por Multiplex PCR y Electroforesis Capilar

Article DOI: 10.3791/59455-v • 10:33 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1,2
1Fish Health Research Group, Norwegian Veterinary Institute, 2University of Bergen

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El ensayo Multi-Locus Variable-number tandem-repeat Analysis (MLVA) presentado aquí permite el genotipado económico, robusto y portátil de alta resolución de la bacteria patógena de peces Yersinia ruckeri. A partir de cultivos puros, el ensayo emplea PCR multiplex y electroforesis capilar para producir perfiles MLVA de diez loci para aplicaciones posteriores.

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Genética Número 148 Yersinia ruckeri yersiniosis MLVA genotipado epidemiología molecular patógeno de peces bacterianos salmón atlántico trucha arco iris
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