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Methods to Test Endocrine Disruption in Drosophila melanogaster
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Developmental Biology
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JoVE Journal Developmental Biology
Methods to Test Endocrine Disruption in Drosophila melanogaster

Methods to Test Endocrine Disruption in Drosophila melanogaster

DOI:
10.3791/59535-v

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09:43 min

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July 03, 2019

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, , , , ,
1Institute of Biosciences and BioResources,National Research Council of Italy (CNR), 2Institute of Research on Terrestrial Ecosystems (IRET),National Research Council of Italy (CNR), 3Department of Biology,UniNa “Federico II”

Chapters

  • 00:04Title
  • 01:01Feeding Assay and Fecundity/Fertility Assay
  • 05:04Developmental Timing Eclosion Assay Protocol 1
  • 06:36Lifespan Protocol
  • 08:25Results: Lifespan Curves
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

Endocrine disruptor chemicals (EDCs) represent a serious problem for organisms and for natural environments. Drosophila melanogaster represents an ideal model to study EDC effects in vivo. Here, we present methods to investigate endocrine disruption in Drosophila, addressing EDC effects on fecundity, fertility, developmental timing, and lifespan of the fly.

Tags

Drosophila MelanogasterEndocrine DisruptionFertilityDevelopmentLifespanEndocrine DisruptorsIn VivoReproductive PerformanceCornmeal MediumEE2Carbon Dioxide

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