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Preparazione del midollo osseo intero per l'analisi della citometria di massa delle cellule di lignaggio neutrofilo
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Preparazione del midollo osseo intero per l'analisi della citometria di massa delle cellule di lignaggio neutrofilo

Article DOI: 10.3791/59617-v • 08:09 min • June 19th, 2019
June 19th, 2019 •
1, 1, 1, 1, 1, 2, 2, 1
1Division of Inflammation Biology, La Jolla Institute for Immunology, 2Flow Cytometry Core Facility, La Jolla Institute for Immunology

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Qui, presentiamo un protocollo per elaborare il midollo osseo fresco (BM) isolato dal topo o dall'uomo per l'analisi di citometria di massa ad alta dimensione (Cytometria da Time-Of-Flight, CyTOF) delle cellule di filo neutrofilo.

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Immunologia e infezione numero 148 Valore vuoto Emissione Cellule di neutrofilo Midollo Osseo BM Citometria di massa CitOF Citometria di flusso viSNE
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