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Journal / Genetics / RNA의 발자국 의 식별:연속RNA 면역 침전을 통한 단백질 복합체 시퀀싱…
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Genetics

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JoVE Journal Genetics
RNA의 발자국 의 식별
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
Journal (Genetics)
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
Journal (Genetics)
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
 
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RNA의 발자국 의 식별:연속RNA 면역 침전을 통한 단백질 복합체 시퀀싱 (RIPiT-Seq)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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여기에서, 우리는 포유류 세포에서 내인성 RNA 결합 사이트 또는 RNA의 "발자국"을 풍부하게 하는 프로토콜을 제시합니다:단백질 (RNP) 복합체. 이 접근은 RNP 소단위의 2개의 면역 강절을 관련시키고 그러므로 나란히 RNA 면역 침전이라고 불립니다 (RIPiT).

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유전학 문제 149 RNA 발자국 RIPiT RNA 결합 단백질 RBP RNA:단백질 복합체 RNP
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