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Journal / Developmental Biology / Vurdering af oxidative skader i den primære mus…
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Developmental Biology
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JoVE Journal Developmental Biology
Vurdering af oxidative skader i den primære mus okulære overfladeceller / stamceller som reaktion på Ultraviolet-C (UV-C) Skader
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Vurdering af oxidative skader i den primære mus okulære overfladeceller / stamceller som reaktion på Ultraviolet-C (UV-C) Skader

Article DOI: 10.3791/59924-v • 12:59 min • February 15th, 2020
February 15th, 2020 •
*1, *1, *1, 1, 1, 1
1Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India
* These authors contributed equally

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Denne protokol viser samtidig påvisning af reaktive iltarter (ROS), levende celler og døde celler i levende primære kulturer fra museoklære overfladeceller. 2',7'-Dichlorfluoresceindiacetate, propidiumiodid og Hoechst farvning anvendes til at vurdere henholdsvis ROS, døde celler og levende celler efterfulgt af billeddannelse og analyse.

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Udviklingsbiologi UV-C primære okulære stamceller reaktive iltarter (ROS) 2',7'-dichlorfluoresceindiacetate (DCFDA) Levende/død celledetektion UV-C-skader
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