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Identification of Circular RNAs using RNA Sequencing
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Genetics
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JoVE Journal Genetics
Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

DOI:
10.3791/59981-v

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08:25 min

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November 14, 2019

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, , , , , ,
1Translational Genomic Research Institute, 2Arizona Alzheimer’s Consortium, 3Banner Sun Health Research Institute

Chapters

  • 00:05Title
  • 01:01RNase R Treatment
  • 01:58RNA Purification
  • 03:41circRNA Library Preparation
  • 06:25Results: Circular RNA Detection
  • 07:39Conclusion

Summary

Automatic Translation

Automatic Translation

Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.

Tags

Circular RNARNA SequencingRNase RRNA CleanupRNA Binding BufferRNA Wash BufferAgilent BioanalyzerTapeStationTotal RNARNA InputProtocolLibrary PreparationCircRNA DetectionSample TypesFunctional Role

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