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JoVE Journal Biochemistry
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Article DOI: 10.3791/60005-v • 06:52 min • November 1st, 2019
November 1st, 2019 •
*1,2,3, *1,2,3, 1,3, 1,3, 1,2,3, 1,2,3
1Department of Physics, Chemistry and Biology, Linköping University, 2Wallenberg Centre for Molecular Medicine (WCMM), 3Nucleic Acids Technologies Laboratory (NAT-lab), Linköping University
* These authors contributed equally

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Summary
Automatic Translation

Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.

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Kinetic Screening Nuclease Activity Nucleic Acid Probes Biomarker Of Disease Probe-nuclease Interaction Flexibility Reproducibility Ease Of Use Oligonucleotide Library DNA Sequence RNA Sequence Adenine Guanine Cytosine Thiamine Uracil Tris-EDTA Buffer Nuclease Degradation Bacterial Culture Solid Medium Porous Glass Bead TSA Supplement Defibrinated Sheep Blood Streaking Colonies Liquid Medium TSB Incubation
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