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Journal / Cancer Research / Obtención de esferas de células madre de cáncer…
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JoVE Journal Cancer Research
Obtención de esferas de células madre de cáncer de cáncer de tumores ginecológicos y de cáncer de mama
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Journal (Cancer Research)
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Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
Journal (Cancer Research)
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
 
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Obtención de esferas de células madre de cáncer de cáncer de tumores ginecológicos y de cáncer de mama

Article DOI: 10.3791/60022-v • 07:01 min • March 1st, 2020
March 1st, 2020 •
*1,2,3,4, *1,2,3,4,5,6, 1,2,3, 2,7, 1,2,3,4,8, 9, 3,9,10, 1,2,3,4,8
1Institute of Biophysics, Faculty of Medicine, University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, 6Gynecology A Service, Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences
* These authors contributed equally

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El objetivo de esta metodología es identificar células madre cancerosas (CSC) en líneas celulares cancerosas y muestras de tumores humanos primarios con el protocolo de formación de esferas, de manera robusta, utilizando ensayos funcionales y caracterización fenotípica con citometría de flujo y occidental Blot.

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Cancer Research Número 157 células madre neoplásicas neoplasias mamarias tumoresferas cáncer ginecológico protocolo de formación de esferas marcadores de células madre de cáncer
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