Journal
/
/
Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing
DOI:

05:45 min

October 25, 2019

, , ,

Chapters

  • 00:04Title
  • 00:38Hypoxic Ventilatory Response (HVR) Recording
  • 02:47Carotid Sinus Nerve Dissection (CSND)
  • 03:37Induction of Leptin Receptor Long Isoform (LepRb) Expression
  • 04:27Results: Representative Effects of Leptin and LepRb Expression on HVR
  • 05:17Conclusion

Summary

Automatic Translation

Our study focuses on the effects of leptin signaling in carotid body (CB) on the hypoxic ventilatory response (HVR). We performed 'loss of function' experiments measuring the effect of leptin on HVR after CB denervation and 'gain of function' experiments measuring HVR after overexpression of the leptin receptor in CB.

Related Videos

Read Article