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Journal / Immunology and Infection / Kultur av liten kolonivariant av Pseudomonas…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Kultur av liten kolonivariant av Pseudomonas aeruginosa och Antal av dess Alginate
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Kultur av liten kolonivariant av Pseudomonas aeruginosa och Antal av dess Alginate

Article DOI: 10.3791/60466-v • 09:06 min • February 22nd, 2020
February 22nd, 2020 •
*1, *2, 1,2,3
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, 2Progenesis Technologies LLC, Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine, Marshall University
* These authors contributed equally

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Här beskriver vi en tillväxt villkor för att kultur den lilla kolonivarianten av Pseudomonas aeruginosa. Vi beskriver också två separata metoder för detektion och mängder av exopolysackarideralginate som produceras av P. aeruginosa med hjälp av en traditionell urinsyra karbazolanalys och en alginate-specifik monoklonal antikropp (mAb) baserad ELISA.

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Immunologi och infektion Utgåva 156 Pseudomonas aeruginosa cystisk fibros (CF) liten koloni variant (SCV) pyrimidin biosyntes tillväxt mucoidy alginate urinsyra karbazol analys alginate-specifika mAb ELISA
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